protease inhibitor cocktail set 1 (Merck KGaA)
90
Structured Review
Merck KGaA
protease inhibitor cocktail set 1
Protease Inhibitor Cocktail Set 1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protease+inhibitor+cocktail+set+1/protease+inhibitor+cocktail+set+iii/pm40632859-273-51-57
Average 90 stars, based on 1 article reviews
Protease Inhibitor Cocktail Set 1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protease+inhibitor+cocktail+set+1/protease+inhibitor+cocktail+set+iii/pm40632859-273-51-57
Average 90 stars, based on 1 article reviews
protease inhibitor cocktail set 1 - by Bioz Stars,
2026-10
90/100 stars
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Western Blot:Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle. Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100 ◦C in 5% boiling SDS with 2 × final concentration of Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2× final concentration of Article Title: Functional diversification of Paramecium Ku80 paralogs safeguards genome integrity during precise programmed DNA elimination Article Snippet: .. Aliquots of ~1.5 x 10 4 frozen concentrated cells were directly lysed following addition of an equal volume of boiling 10% SDS containing Incubation:Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle. Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100 ◦C in 5% boiling SDS with 2 × final concentration of Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2× final concentration of Article Title: Functional diversification of Paramecium Ku80 paralogs safeguards genome integrity during precise programmed DNA elimination Article Snippet: .. Aliquots of ~1.5 x 10 4 frozen concentrated cells were directly lysed following addition of an equal volume of boiling 10% SDS containing Article Title: Down-regulation of human-specific lncRNA TMEM9B-AS1 in skeletal muscle of people with type 2 diabetes affects ribosomal biogenesis. Article Snippet: .. Puromycin (1 μM final) was then added to the media and incubated for 30 min. Before the harvest, the cells were washed with ice- cold PBS and scraped into lysis buffer consisting of 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% (v/v) Triton X- 100, 10% (v/v) glycerol, 20 mM tris (pH 7.8), 1 mM EDTA, 1 mM PMSF, 1% ( Concentration Assay:Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle. Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100 ◦C in 5% boiling SDS with 2 × final concentration of Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2× final concentration of Protease Inhibitor:Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle. Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100 ◦C in 5% boiling SDS with 2 × final concentration of Article Title: Inter-generational nuclear crosstalk links the control of gene expression to programmed genome rearrangement during the Paramecium sexual cycle Article Snippet: .. For western blots, 20 μl of frozen cells were lysed by a 3-min incubation at 100°C in 5% boiling SDS with 2× final concentration of Article Title: Down-regulation of human-specific lncRNA TMEM9B-AS1 in skeletal muscle of people with type 2 diabetes affects ribosomal biogenesis. Article Snippet: The cells were transfected with control stealth RNA interference (RNAi) (control #1, Medium GC, Invitrogen) or with TMEM9B- AS1 stealth RNAi (HSS162366, Thermo Fisher Scientific; https://www.thermofisher.com/order/genome- database/details/sirna/ HSS162366?CID=&ICID=&subtype=sirna_stealth). .. Then, 10 days after the start of differentiation, the cells were scraped and harvested in 300 μl of IP lysis buffer [25 mM tris- HCl (pH 7.4), 150 mM NaCl, 1% NP- 40, 1 mM EDTA, and 5% glycerol], supplemented with Article Title: Functional diversification of Paramecium Ku80 paralogs safeguards genome integrity during precise programmed DNA elimination Article Snippet: .. Aliquots of ~1.5 x 10 4 frozen concentrated cells were directly lysed following addition of an equal volume of boiling 10% SDS containing Article Title: Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism Article Snippet: Paired FDB muscles were placed in KHB containing 5 mM glucose and 15 mM mannitol for a 20-min-period before being subjected to either electrical stimulation (100 Hz, 0.2 ms pulse duration, ~20 V, one contraction of 0.2 s every 2 s) or rest condition for 10 min. After contraction or insulin-stimulation, muscles were incubated in KHB supplemented with 20 mM mannitol for 10 min before incubation in KHB supplemented with 1 mM 2-deoxyglucose and [ 3 H]2-deoxy-glucose (2.5 μCi mL −1 , American Radiolabeled Chemicals, MO) and 19 mM mannitol and [ 14 C]mannitol (0.7 mCi mL −1 , Moravek Inc., CA) for 20 min. .. Thereafter, muscles were stripped of connective tissue and snap frozen in liquid nitrogen and later homogenized in homogenization buffer (137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , 0.5 mM Na 3 VO 4 , 1% (vol/vol) Triton X-100, 10% (vol/vol) glycerol, 20 mM Tris, 10 mM NaF, 1 mM EDTA, 1 mM PMSF, and 1% ( Article Title: Down-regulation of human-specific lncRNA TMEM9B-AS1 in skeletal muscle of people with type 2 diabetes affects ribosomal biogenesis. Article Snippet: .. The cells were then lysed and homogenized using a lysis buffer consisting of 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.5 mM Na3VO4, 1% (v/v) Triton X- 100, 10% (v/v) glycerol, 20 mM tris (pH 7.8), 1 mM EDTA, with the addition of 1 mM phenylmethylsulfonyl fluoride (PMSF), 1% ( Article Title: Down-regulation of human-specific lncRNA TMEM9B-AS1 in skeletal muscle of people with type 2 diabetes affects ribosomal biogenesis. Article Snippet: .. Puromycin (1 μM final) was then added to the media and incubated for 30 min. Before the harvest, the cells were washed with ice- cold PBS and scraped into lysis buffer consisting of 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% (v/v) Triton X- 100, 10% (v/v) glycerol, 20 mM tris (pH 7.8), 1 mM EDTA, 1 mM PMSF, 1% ( Article Title: Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism Article Snippet: .. Cells and tissues were lysed in ice-cold homogenization buffer (137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , 0.5 mM Na 3 VO 4 , 1% (vol/vol) Triton X-100, 10% (vol/vol) glycerol, 20 mM Tris, 10 mM NaF, 1 mM EDTA, 1 mM PMSF, and 1% ( Lysis:Article Title: Down-regulation of human-specific lncRNA TMEM9B-AS1 in skeletal muscle of people with type 2 diabetes affects ribosomal biogenesis. Article Snippet: The cells were transfected with control stealth RNA interference (RNAi) (control #1, Medium GC, Invitrogen) or with TMEM9B- AS1 stealth RNAi (HSS162366, Thermo Fisher Scientific; https://www.thermofisher.com/order/genome- database/details/sirna/ HSS162366?CID=&ICID=&subtype=sirna_stealth). .. Then, 10 days after the start of differentiation, the cells were scraped and harvested in 300 μl of IP lysis buffer [25 mM tris- HCl (pH 7.4), 150 mM NaCl, 1% NP- 40, 1 mM EDTA, and 5% glycerol], supplemented with Article Title: Down-regulation of human-specific lncRNA TMEM9B-AS1 in skeletal muscle of people with type 2 diabetes affects ribosomal biogenesis. Article Snippet: .. The cells were then lysed and homogenized using a lysis buffer consisting of 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.5 mM Na3VO4, 1% (v/v) Triton X- 100, 10% (v/v) glycerol, 20 mM tris (pH 7.8), 1 mM EDTA, with the addition of 1 mM phenylmethylsulfonyl fluoride (PMSF), 1% ( Article Title: Down-regulation of human-specific lncRNA TMEM9B-AS1 in skeletal muscle of people with type 2 diabetes affects ribosomal biogenesis. Article Snippet: .. Puromycin (1 μM final) was then added to the media and incubated for 30 min. Before the harvest, the cells were washed with ice- cold PBS and scraped into lysis buffer consisting of 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1% (v/v) Triton X- 100, 10% (v/v) glycerol, 20 mM tris (pH 7.8), 1 mM EDTA, 1 mM PMSF, 1% ( Muscles:Article Title: Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism Article Snippet: Paired FDB muscles were placed in KHB containing 5 mM glucose and 15 mM mannitol for a 20-min-period before being subjected to either electrical stimulation (100 Hz, 0.2 ms pulse duration, ~20 V, one contraction of 0.2 s every 2 s) or rest condition for 10 min. After contraction or insulin-stimulation, muscles were incubated in KHB supplemented with 20 mM mannitol for 10 min before incubation in KHB supplemented with 1 mM 2-deoxyglucose and [ 3 H]2-deoxy-glucose (2.5 μCi mL −1 , American Radiolabeled Chemicals, MO) and 19 mM mannitol and [ 14 C]mannitol (0.7 mCi mL −1 , Moravek Inc., CA) for 20 min. .. Thereafter, muscles were stripped of connective tissue and snap frozen in liquid nitrogen and later homogenized in homogenization buffer (137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , 0.5 mM Na 3 VO 4 , 1% (vol/vol) Triton X-100, 10% (vol/vol) glycerol, 20 mM Tris, 10 mM NaF, 1 mM EDTA, 1 mM PMSF, and 1% ( Homogenization:Article Title: Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism Article Snippet: Paired FDB muscles were placed in KHB containing 5 mM glucose and 15 mM mannitol for a 20-min-period before being subjected to either electrical stimulation (100 Hz, 0.2 ms pulse duration, ~20 V, one contraction of 0.2 s every 2 s) or rest condition for 10 min. After contraction or insulin-stimulation, muscles were incubated in KHB supplemented with 20 mM mannitol for 10 min before incubation in KHB supplemented with 1 mM 2-deoxyglucose and [ 3 H]2-deoxy-glucose (2.5 μCi mL −1 , American Radiolabeled Chemicals, MO) and 19 mM mannitol and [ 14 C]mannitol (0.7 mCi mL −1 , Moravek Inc., CA) for 20 min. .. Thereafter, muscles were stripped of connective tissue and snap frozen in liquid nitrogen and later homogenized in homogenization buffer (137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , 0.5 mM Na 3 VO 4 , 1% (vol/vol) Triton X-100, 10% (vol/vol) glycerol, 20 mM Tris, 10 mM NaF, 1 mM EDTA, 1 mM PMSF, and 1% ( Article Title: Endurance exercise training-responsive miR-19b-3p improves skeletal muscle glucose metabolism Article Snippet: .. Cells and tissues were lysed in ice-cold homogenization buffer (137 mM NaCl, 2.7 mM KCl, 1 mM MgCl 2 , 0.5 mM Na 3 VO 4 , 1% (vol/vol) Triton X-100, 10% (vol/vol) glycerol, 20 mM Tris, 10 mM NaF, 1 mM EDTA, 1 mM PMSF, and 1% ( |